de Rougemont A, Kaplon J, Billaud G, Lina B, Pinchinat S, Derrough T, Caulin E, Pothier P, Floret D.

Sensitivity and specificity of the VIKIA® Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIATM Rotavirus kit (Dako) compared to genotyping. Pathol Biol. 2008; 56 :1-4.

Centre national de référence des virus entériques, laboratoire de virologie, plateau technique de biologie, CHU de Dijon, 2, rue Angélique-Ducoudray, BP 37013, 21070 Dijon cedex, France.

The performances of two diagnostic tests for rotavirus infection in stool samples were evaluated during a prospective study in children of less than 36 months in child-care centers of Lyon from November 2004 to May 2005. The VIKIA((R)) Rota-Adeno immuno-chromatographic test (bioMérieux) and the ELISA IDEIAtrade mark Rotavirus kit (Dako) were compared with a referral method, the genotyping. Fifty-seven stool samples were collected and analyzed by RT-PCR. The virus genome was detected in 29 samples. The most frequent genotypic combinations were G9P[8] with a prevalence of 75.9%. Sensitivity and specificity of the VIKIA((R)) Rota-Adeno test and the ELISA IDEIAtrade mark Rotavirus kit were strictly comparable and very good: 96.6% (83.0; 99.9) and 96.4% (81.6; 99.9), respectively. The immuno-chromatographic technique were in concordance with the ELISA tests in 93.6% of cases. Thus, the VIKIA((R)) Rota-Adeno test is a good alternative for the occasional analysis of stool samples in ambulatory practice.